The Acetyl form at the end of Link reaction is picked up by the COA (Co enzyme A) present in the matrix to form acetyl COA, then the acetyl combine with Oxaloacetate of 4C acid to form a 6C compound citrate, the COA leaves and it can pick another acetyl to bring into the Krebs Cycle, The citrate undergoes decarboxylation and dehydrogenation to yield carbon dioxide NAD molecule, 5C compounds formed during a series of reaction, An important intermediate Alpha Ketoglutrate is also formed into the Krebs Cycle which is a 5C compound. After this stage the dehydrogenation reaction occur trice resulting in two reduced NAD and 1 reduced FAD. 1 molecule of ATP is also produced during the rearrangement of the substrate.
This ATP formation like that of formed during glycolysis is termed as substrate level Phosphorylation. Decarboxylation results at this state in the complete breakdown of acetyl and the 4C compound Oxaloacetate regenerated to again start a fresh Krebs Cycle.
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